CAR-T Cell Therapy Testing

A rapidly emerging immunotherapy approach is called adoptive cell transfer (ACT): collecting and using patients’ own immune cells to treat their cancer.

Services provided for CAR-T Edited Cell Screening

  • Screening primers are designed to create an amplicon over the targeted region of pooled DNA
  • Typically the amplicon length of 200 bps maximizes detection of a variety of mutations ensuring the majority of the reads reach the forward and reverse barcodes
  • Unique barcoded amplicons are produced with up to 96 clones/plate using 12 barcoded forward primers and 8 barcoded reverse primers
  • The amplicons are PCR amplification and clones pooled
  • Adapters are ligated to the clone pools
  • A library is created of the fragment pools
  • The library is sequenced with NGS
  • Gapped mapping is employed to identify indels
  • A report is prepared and delivered

Our services include:

  • Methods validated per ICH GLP/GMP
  • Research grade testing also available
  • Projects can be tailored to suit your needs
  • Professional project reports that include raw and analyzed data
  • Competitive pricing
  • Fast turn-around time
  • Weekly progress reports
 

 

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We are dedicated to timely, high-quality service from the start of your project to the finish. Send us a quick message with your questions to find out what Avance can do for you.

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Give us a little background on your needs and the goals of your project, and we’ll send a preliminary quote including: tasks, deliverables and a no-obligation cost estimate.