Custom DNA sequencing

The following custom DNA sequencing services are offered in Avance Biosciences based on the type of samples to be sequenced and/or the techniques utilized. Avance Biosciences is a “custom” sequencing company and is specialized in offering a complete solution to customer needs.

Plasmid Sequencing

Plasmid DNAs are separated from cells and used as sequencing template to delineate the sequence of either the whole plasmid or that of a gene(s) of interest.

Amplicon Sequencing

PCR products of same gene(s) of interest on a cohort of samples are generated, purified, and sequenced. After analysis, sequence variations, such as SNPs, can be reported.

mRNA/cDNA Sequencing

RNA extracted from cell or tissue will be converted to cDNA, PCR amplified and sequenced for the purpose of verifying expressed transcript of a gene(s) of interest.

Shotgun Sequencing

Genomic DNA, or any DNA of large length, is randomly fragmented and then sub-cloned into a cloning vector. The subclones are random picked and sequenced. The sequence reads are then assembled to form contigs.

Primer-walking

DNA sequencing is delineated through iterative rounds of primer design and sequencing.

Viral DNA/RNA Sequencing

Viral DNAs or RNAs are separated from viral particles followed by PCR or RT-PCR before sequencing reactions are performed.

Contact our customer service to discuss your sequencing project needs.

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