Sanger sequencing or the chain termination method or capillary-based sequencing is based on the selective incorporation of chain-terminating dideoxynucleotides by DNA polymerase. Currently, Sanger sequencing is used for plasmids, viral sequencing, confirmation of cloning structures, etc.
The FDA continues to require the completion of two-fold double stranded sequencing for identity and safety testing of products produced from cell lines via integrated or non-integrated vectors because DNA/RNA-genetic sequences are susceptible to random mutation.
With more than twenty years of experience and sequencing knowledge, Avance Biosciences experienced scientists can execute DNA sequencing techniques of all complexities including difficult templates, viral, bacterial and genomic DNA, as well as projects tailored to your needs.
Genomics & Biological Services
All Avance Services Include
Experienced scientists functioning as project managers or study directors
Controlled laboratory environment to prevent cross-contamination
Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination
Methods validated per ICH GLP/GMP guidelines – also research grade testing available
Projects customized and tailored to suit your needs
Independent quality unit assuring regulatory compliance
Transparent process flow and weekly report on project progress
Professional project reports ready for regulatory filing
Fast turn-around time & Competitive pricing