Avance Biosciences™ offers Sanger sequencing to support our clients worldwide for their drug development and manufacturing activities. The FDA continues to require the completion of two-fold double stranded Sanger sequencing for genomic identity of biologics produced from cell lines via integrated or non-integrated vectors. Avance Biosciences™ experienced scientists can execute DNA sequencing techniques of all complexities including difficult templates, viral, bacterial and genomic DNA, as well as projects tailored to your needs
Plasmid Sequencing |
Plasmid DNAs are separated from cells and used as sequencing template to delineate the sequence of either the whole plasmid or that of a gene(s) of interest. |
Amplicon Sequencing | PCR products of same gene(s) of interest on a cohort of samples are generated, purified, and sequenced. After analysis, sequence variations, such as SNPs, can be reported. |
mRNA/cDNA Sequencing | RNA extracted from cell or tissue will be converted to cDNA, PCR amplified and sequenced for the purpose of verifying expressed transcript of a gene(s) of interest |
Shotgun Sequencing | Genomic DNA, or any DNA of large length, is randomly fragmented and then sub-cloned into a cloning vector. The subclones are random picked and sequenced. The sequence reads are then assembled to form contigs. |
Primer-Walking | DNA sequencing is delineated through iterative rounds of primer design and sequencing. |
Viral DNA/RNA Sequencing | Viral DNAs or RNAs are separated from viral particles followed by PCR or RT-PCR before sequencing reactions are performed. |
Our Customers Say…
From the preliminary conclusion of this work, we are already pleased to thank you for your customer-oriented approach and proactive communication in the course of this study. We are happy to see the profound difference in business mindset between your organization and our previous vendor…
Project Manager, Belgium
Thank you so much for successfully completing this important project for us on time. We are very satisfied with the final report and thank you for taking the extra effort to customize the report format. We will definitely use your lab for future projects.
Program Manager, California
It has been a pleasure working with you and your team. The project was carried out to the highest standard and delivered in a timely manner. I will definitely recommend Avance Biosciences™’ services to colleagues and friends.
Senior Scientist, Singapore