This high quality cGMP/GLP DNA and mRNA sequencing service is offered to satisfy regulatory requirements of identity and purity of cell lines used in drug development and manufacturing. Service is also offered at research grade.
Avance Biosciences will perform bi-directional DNA sequencing analysis for the expressed gene of interest using ABI Prism™ 3730xl DNA sequencers. Total RNA will be extracted from the test article followed by spectrophotometry quantitation and gel electrophoresis analysis. PCR and sequencing primers will be designed as needed for overlapping coverage. Extracted RNA will be reverse transcribed and then PCR amplified. Double stranded DNA sequencing reactions will be performed and sequencing results will be analyzed. The consensus sequence derived will be compared to the reference sequence provided by client.
Genomics & Biological Services
All Avance Services Include
Experienced scientists functioning as project managers or study directors
Controlled laboratory environment to prevent cross-contamination
Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination
Methods validated per ICH GLP/GMP guidelines – also research grade testing available
Projects customized and tailored to suit your needs
Independent quality unit assuring regulatory compliance
Transparent process flow and weekly report on project progress
Professional project reports ready for regulatory filing
Fast turn-around time & Competitive pricing