Regulatory agencies require genetic stability tests on cell banks used for biologicals development and manufacturing. Avance Biosciences offers a series of molecular and microbial assays for testing the genetic stability of Master Cell Banks (MCB), Working Cell Banks (WCB) and End of Production Cells (EOPC).

Sequencing of
Expression Construct

High quality cDNA sequencing with a 2-fold coverage on both DNA strand is offered as part of cell line identity and stability tests.

Copy Number
Variation Analysis

This service determines the average copy number of a plasmid in a cell. The ABI 7900HT Sequence Detection System is used to measure the quantities of the plasmid and the host DNA present in a cell line sample.

Structure Analysis
by Southern Blot

Restriction enzyme digestion of cell line DNA followed by Southern blotting is used to evaluate genetic stability of the integration site of an expression construct.

Lytic and Lysogenic
Phage Detection

Services are designed to test the presence or absence of lytic phage and prophage contaminants in E. coli bacterial cell banks. –  Application Note – Phage Testing of Bacterial Cultures

Transcript Analysis
by Northern Blot

This service is offered to detect the presence of an expressed transcript containing a sequence of interest. The size of the transcript will be estimated and the presence of partial transcripts will be reported.

Marker Retention/Plasmid loss
by Antibiotic Resistance

This service is offered to determine the percentage of cells in a cell line sample that contain a selectable marker such as antibiotic resistance.

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Our Customers Say…

From the preliminary conclusion of this work, we are already pleased to thank you for your customer-oriented approach and proactive communication in the course of this study. We are happy to see the profound difference in business mindset between your organization and our previous vendor…

Project Manager, Belgium

Thank you so much for successfully completing this important project for us on time. We are very satisfied with the final report and thank you for taking the extra effort to customize the report format. We will definitely use your lab for future projects.

Program Manager, California

It has been a pleasure working with you and your team. The project was carried out to the highest standard and delivered in a timely manner. I will definitely recommend Avance Biosciences’ services to colleagues and friends.

Senior Scientist, Singapore

When you partner with Avance Biosciences, you gain a CRO partner that is creative, collaborative and dedicated to sound science with a focus on the regulatory requirements our partners require. Contact our technical staff to discuss how we can support you in your project!

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Dedication to Quality

Dedication To Quality

The Avance Biosciences team is committed to strict adherence to CGMP and GLP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

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Extensive Experience

Open Communication

Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

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Open Communication

Open Communication

Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

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Cutting Edge Science

Open Communication

Avance Biosciences’ CGMP/GLP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, ddPCR, Sanger sequencing, next-gen sequencing, Southern blot, and more.

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