E. coli Cell Bank Characterization Services

Ensure identity, purity, and genetic stability of E. coli cell banks with GMP-compliant testing for plasmid and protein production.

Meet regulatory requirements with validated assays that confirm contaminant-free, stable cell lines for safe and reliable biomanufacturing.

E. coli cell banks serve as the foundation for the biomanufacturing of plasmids and recombinant proteins, offering a fast, scalable, and cost-effective platform for therapeutic production. Avance Biosciences offers comprehensive E. coli cell bank testing services, including: pre-bank screening, CGMP-compliant release testing, and advanced molecular assays to ensure identity, purity, stability, and regulatory compliance at every stage of biomanufacturing.

E. coli Pre-bank Testing

Phage Detection: Microbiological assays to identify lytic and lysogenic phage contamination.
Plasmid Sequencing: Full or partial plasmid sequence confirmation using Sanger or NGS.
Phage ID by NGS: Accurate identification of phage species through next-generation sequencing.

E. coli Cell Bank Testing (CGMP)

Identity & Purity Testing: Comprehensive assays including strain ID, species confirmation, Gram staining, and purity testing for bacteria and fungi.
Genetic Stability Analysis: Plasmid sequence confirmation, gene of interest validation, and plasmid copy number via qPCR or ddPCR.
Cell Bank Quality Assurance: Lytic/lysogenic phage detection, marker retention, viability testing, and mRNA sequencing for robust cell bank characterization.

Other E. coli Cell Bank Testing

Antibiotic Sensitivity Testing: Determine resistance profiles to ensure safe and effective bioprocessing.
Microbial Species Identification: Identify bacterial or fungal species using 16S/ITS sequencing.
Genome & Plasmid Verification: Confirm E. coli genome and residual plasmid clearance with NGS and qPCR assays.

Our Customers Say…

Your team met the timelines we needed and did exceptional technical work. I will definitely make my management aware that Avance is a very capable partner for our bioanalytical needs.

Bioanalytical Senior Project Manager, California

Avance Biosciences™ (AB) has delivered the Sequencing data in a record time. Very impressive! We have benefited from AB’s hard work and are very grateful for that. AB team should feel proud of themselves. We thank AB team for their support and contributions, much appreciated.

Head of Quality Control, Microbial Manufacturing Service, California

My experience with Avance has been nothing but positive… I will keep sending work your way as I appreciate the attention your team provides and Avance’s creative technical team. See you soon.

Director Individualized Medicines, California

Avance has been the ideal partner in helping Mission Bio and our client,… be the first in the world to GMP qualify our single cell % transduction assay for a lentiviral gene therapy product. Excellent execution on the part of the Avance team. We look forward to more collaborations in the future.

Senior Director, Cell & Gene Therapy Business, California

From the preliminary conclusion of this work, we are already pleased to thank you for your customer-oriented approach and proactive communication in the course of this study. We are happy to see the profound difference in business mindset between your organization and our previous vendor…

Project Manager, Belgium

Thank you so much for successfully completing this important project for us on time. We are very satisfied with the final report and thank you for taking the extra effort to customize the report format. We will definitely use your lab for future projects.

Program Manager, California

It has been a pleasure working with you and your team. The project was carried out to the highest standard and delivered in a timely manner. I will definitely recommend Avance Biosciences™’ services to colleagues and friends.

Senior Scientist, Singapore