Amplicon sequencing is a highly targeted approach that enables researchers to analyze genetic variation in specific genomic regions. The ultra-deep sequencing of PCR products (amplicons) allows efficient variant identification, characterization and quantitation.

Avance offers multiple NGS amplicon-based targeted sequencing approaches with a focus on scalability, speed, and cost using the state-of-the-art Illumina MiSeq next-gen sequencing platform. We provide GLP/GMP compliant assay development, assay validation, and sample testing services for several amplicon sequencing applications in support of biological drug development:

  • Targeted genome resequencing

  • Deep sequencing for rare allelic variants

  • Mutation analysis for prepared cell lines

  • CRISPR Edited gene characterization

  • 16S rRNA gene sequencing

  • Preclinical biodistribution studies

  • Edited gene quantitation for pharmacokinetics studies

  • SNP genotyping

  • Cancer panel sequencing

Comprehensive Amplicon
Sequencing Workflow

DNA

DNA
Extraction

QPCR

PCR
Amplification

MiSeq

Next-Gen
Sequencing

Data
Analysis

Validation

Customer
Report

Have questions?

We are dedicated to timely, high-quality service from the start of your project to the finish. Send us a note or give us a call to find out what Avance can do for you.

SEND US A NOTE

Ready to get started?

Give us a little background on your needs and the goals of your project, and we’ll send a preliminary quote including: tasks, deliverables and a no-obligation cost estimate.

REQUEST A QUOTE

Our Customers Say…

From the preliminary conclusion of this work, we are already pleased to thank you for your customer-oriented approach and proactive communication in the course of this study. We are happy to see the profound difference in business mindset between your organization and our previous vendor…

Project Manager, Belgium

Thank you so much for successfully completing this important project for us on time. We are very satisfied with the final report and thank you for taking the extra effort to customize the report format. We will definitely use your lab for future projects.

Program Manager, California

It has been a pleasure working with you and your team. The project was carried out to the highest standard and delivered in a timely manner. I will definitely recommend Avance Biosciences’ services to colleagues and friends.

Senior Scientist, Singapore

When you partner with Avance Biosciences, you gain a CRO partner that is creative, collaborative and dedicated to sound science with a focus on the regulatory requirements our partners require. Contact our technical staff to discuss how we can support you in your project!

Partner

Dedication to Quality

Dedication To Quality

The Avance Biosciences team is committed to strict adherence to GLP and CGMP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

Extensive Experience

Extensive Experience

Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

Open Communication

Open Communication

Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

Cutting Edge Science

Open Communication

Avance Biosciences’ GLP/GMP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, digital PCR, Sanger sequencing, next generation sequencing, and more.