Transcript Analysis by Northern Blot service is offered to detect the presence of an expressed transcript containing a sequence of interest. The size of the transcript will be estimated and the presence of partial transcripts will be reported.
Total RNA will be isolated from the test article (integrated cells) and untransfected cells. The total RNA (approximately 2 – 5 µg per sample) will be electrophoresed and transferred/cross-linked to positive-charged nylon membrane. The same probe designed for the Southern blots (if Southern blot analysis is performed) will be used for the Northern blots hybridization. Hybridized transcript(s) will be visualized by exposure of X-Ray film. After producing the autoradiogram, the size of the detected transcript(s) will be determined. A report with northern blot image and film, and transcript sizes will be delivered.
Genomics & Biological Services
All Avance Services Include
Experienced scientists functioning as project managers or study directors
Controlled laboratory environment to prevent cross-contamination
Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination
Methods validated per ICH GLP/GMP guidelines – also research grade testing available
Projects customized and tailored to suit your needs
Independent quality unit assuring regulatory compliance
Transparent process flow and weekly report on project progress
Professional project reports ready for regulatory filing
Fast turn-around time & Competitive pricing