At Avance Biosciences, we are constantly expanding our capabilities to meet the evolving needs of our clients in biologics development and gene and cell therapy. One of our most recent additions is the PacBio HiFi long-read sequencing platform, now fully integrated into our suite of services. But why did we choose to bring this technology online, and how does it benefit plasmid quality control (QC)?
Plasmids are foundational tools in gene therapy, vaccine development, and molecular biology. Ensuring their sequence integrity is critical, as even minor errors or contaminants can impact therapeutic safety, efficacy, and regulatory compliance. Traditional short-read and Sanger sequencing methods are reliable for many applications but can struggle with certain sequence features, such as GC-rich regions, repetitive elements, and complex secondary structures. These are precisely the regions where precision is most needed.
PacBio HiFi technology offers highly accurate, long-read sequencing without the need for PCR amplification, making it ideal for plasmid QC. Its ability to read full-length plasmids in a single pass allows us to detect subtle variants, unintended DNA species, or structural anomalies that might be missed by other sequencing methods. This ensures that our clients can move forward with confidence, knowing that their plasmid sequences are thoroughly validated and compliant with regulatory standards.
By adding PacBio HiFi to our portfolio alongside Illumina, AVITI™, and Oxford Nanopore platforms, Avance Biosciences now offers a comprehensive sequencing toolkit. Whether it’s plasmids, viral vectors, mRNA therapeutics, or complex genetic constructs, we provide the accuracy and detail required to support robust CMC testing and accelerate drug development programs.
In short, PacBio HiFi enhances our ability to provide the high-resolution, high-accuracy plasmid QC that modern biologics development demands, helping ensure safety, reliability, and success in every project.
Accelerate your drug development with Avance Biosciences’ Next-Generation Sequencing (NGS) Center of Excellence, delivering GMP- and GLP-compliant genomics solutions to power your development programs. Our capabilities include Short-read Next-gen Sequencing, Nanopore Third-gen Sequencing, PacBio Third-gen Sequencing, 10X Chromium Single-cell Sequencing, and Mission Bio Tapestri Single-cell Sequencing. Learn more at our NGS Center of Excellence and contact our experts to get started.
