Digital PCR

Digital PCR (dPCR) provides precise and absolute quantification of nucleic acids without a standard curve and without dependence on amplification efficiency.

Digital PCR is ultrasensitive; therefore, appropriate for testing allelic variants and targets of low abundance that are below the sensitivity limit of QPCR. It is also very precise, and is a better tool than QPCR when precise determination of the ratio of several nucleic acid sequences is required.

By employing multiple wells per sample, millions of droplets can be analyzed. Samples are partitioned into ‘droplets’ then large quantities (~20,000 of these partitioned droplets are analyzed using microfluidics technology to provide incomparable precision). Only the positive fraction of the sample is detected; therefore, dPCR can detect mutant alleles in circulating tumor DNA, events as few as 0.01% of cells such as L1 insertions, low-level pathogens, and relative expression in single cells.

Services

cGMP/GLP Compliant
Genomics & Biological Services

Avance Biosciences offers digital PCR services using Bio-Rad’s state-of-the-art Q200 digital droplet PCR system to support clients around the world.  We are specialized in assay development, validation, and sample testing to support GLP and cGMP submissions.
Digital PCR

Digital PCR Applications

  • Cancer biomarker discovery

  • Infectious diseases

  • Genomic alterations

  • Copy number variant analysis

  • Point mutations

  • Rare variant detection

  • Gene expression analysis

  • SNP genotyping

  • Pathogen detection and load determination

  • Absolute quantification of standards

  • Library quantification or clonal amplification of samples for next-generation sequencing

  • Characterization of low-fold changes in mRNA and miRNA expression

  • GMO detection and contamination assessment

When you partner with Avance Biosciences, we give you undivided attention, share our experience and knowledge, and respond to your needs in a timely manner. Contact our technical staff to discuss how we can support you in your project!

Partner

Dedication to Quality

Dedication To Quality

The Avance Biosciences team is committed to strict adherence to cGMP and GLP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

LEARN MORE

Extensive Experience

Open Communication

Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

LEARN MORE

Open Communication

Open Communication

Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

LEARN MORE

Cutting Edge Science

Open Communication

Avance Biosciences’ cGMP/GLP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, ddPCR, Sanger sequencing, next-gen sequencing, Southern blot, and more.

LEARN MORE