Digital PCR (dPCR) provides precise and absolute quantification of nucleic acids without a standard curve and without dependence on amplification efficiency.
Digital PCR is ultrasensitive; therefore, appropriate for testing allelic variants and targets of low abundance that are below the sensitivity limit of QPCR. It is also very precise, and is a better tool than QPCR when precise determination of the ratio of several nucleic acid sequences is required.
By employing multiple wells per sample, millions of droplets can be analyzed. Samples are partitioned into ‘droplets’ then large quantities (~20,000 of these partitioned droplets are analyzed using microfluidics technology to provide incomparable precision). Only the positive fraction of the sample is detected; therefore, dPCR can detect mutant alleles in circulating tumor DNA, events as few as 0.01% of cells such as L1 insertions, low-level pathogens, and relative expression in single cells.
AVANCE IS COMMITTED TO QUALITY
Our industry-leading quality system is designed and maintained to support drug and device research and development, GLP pre-clinical trials, cGMP biomanufacturing, and clinical trials.
Avance Biosciences provides biological testing services to support regulatory submission for clients around the world. These services are conducted under the requirements of Good Laboratory Practices and Current Good Manufacturing Practices as promulgated by the U.S. Food and Drug Administration 21 CFR Part 58 and 21 CFR Part 210 and 211, as well as Good Manufacturing Practice according to ICH Q7, where applicable to the laboratory services provided.
Avance Biosciences offers digital PCR services using Bio-Rad’s state-of-the-art Q200 digital droplet PCR system to support clients around the world. We are specialized in assay development, validation, and sample testing to support GLP and cGMP submissions.
Digital PCR Applications
Cancer biomarker discovery
Copy number variant analysis
Rare variant detection
Gene expression analysis
Pathogen detection and load determination
Absolute quantification of standards
Library quantification or clonal amplification of samples for next-generation sequencing
Characterization of low-fold changes in mRNA and miRNA expression
GMO detection and contamination assessment