Sanger sequencing, referred to as the chain termination method, is a DNA sequencing method based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase during in vitro DNA replication. Avance Biosystems offers many types of sequencing using the ABI 3730 XL DNA Analyzer platform and BigDye v3.1 chemistry.

Sanger sequencing is a “Gold Standard” platform and is used for sequencing clinical trial samples and for validating assays to be used in pre-clinical and clinical trials and to validate nucleic acid based diagnostic tools/devices for PMA or 510K clearances.

  • DNA extraction and QC from all sources

  • 96-well high-throughput plate sequencing

  • Primer design

  • Plasmid preparation

  • Single-stranded (2-fold) or double-stranded (4-fold) coverage per base pair depending on need

  • Manual editing of chromatograms

  • Electropherograms

  • Report containing methods and results

  • Sequence data assembly with comparison, sequence overview, restriction map, and alignments

ABI 3730

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The Avance Biosciences team is committed to strict adherence to cGMP and GLP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

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Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

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Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

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Avance Biosciences’ cGMP/GLP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, ddPCR, Sanger sequencing, next-gen sequencing, Southern blot, and more.

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