Avance Biosciences, Inc. provides all necessary expertise for Southern or Northern blot projects from nucleotide extraction to probe design. We offer Southern blot analysis to support gene discovery and confirmation, gene mapping, evolution and development studies, diagnostics, and forensics tests. Transcript Analysis by Northern Blot service is offered to detect the presence of an expressed transcript containing a sequence of interest.

Quality

AVANCE IS COMMITTED TO QUALITY

Our industry-leading quality system is designed and maintained to support drug and device research and development, GLP pre-clinical trials, cGMP biomanufacturing, and clinical trials.

Avance Biosciences provides biological testing services to support regulatory submission for clients around the world. These services are conducted under the requirements of Good Laboratory Practices and Current Good Manufacturing Practices as promulgated by the U.S. Food and Drug Administration 21 CFR Part 58 and 21 CFR Part 210 and 211, as well as Good Manufacturing Practice according to ICH Q7, where applicable to the laboratory services provided.

We welcome current and potential clients to conduct on-site quality and technical audits. Clients may contact our Quality Unit to schedule a visit.

Southern Blot

Southern Blotting

DNA will be extracted from testing samples followed by UV quantitation and electrophoresis analysis. After restriction digest with enzymes, DNA samples are separated by agarose gel electrophoresis and then transferred and UV cross-linked to a positive charged nylon membrane. Probe DNA will be prepared and labeled with either Chemiluminescent or 32P chemistry. After hybridization, the captured fragments will be visualized by exposure of X-Ray film.

Northern Blotting

Total RNA will be isolated from the test article (integrated cells) and untransfected cells. The total RNA (approximately 2 – 5 µg per sample) will be electrophoresed and transferred/cross-linked to positive-charged nylon membrane. The same probe designed for the Southern blots (if Southern blot analysis is performed) will be used for the Northern blots hybridization. Hybridized transcript(s) will be visualized by exposure of X-Ray film. After producing the autoradiogram, the size of the detected transcript(s) will be determined.

When you partner with Avance Biosciences, we give you undivided attention, share our experience and knowledge, and respond to your needs in a timely manner. Contact our technical staff to discuss how we can support you in your project!

Partner

Dedication to Quality

Dedication To Quality

The Avance Biosciences team is committed to strict adherence to cGMP and GLP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

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Extensive Experience

Open Communication

Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

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Open Communication

Open Communication

Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

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Cutting Edge Science

Open Communication

Avance Biosciences’ cGMP/GLP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, ddPCR, Sanger sequencing, next-gen sequencing, Southern blot, and more.

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