Digital PCR (dPCR) provides precise and absolute quantification of nucleic acids without a standard curve and without dependence on amplification efficiency.

Digital PCR is ultrasensitive; therefore, appropriate for testing allelic variants and targets of low abundance that are below the sensitivity limit of QPCR. It is also very precise, and is a better tool than QPCR when precise determination of the ratio of several nucleic acid sequences is required.

By employing multiple wells per sample, millions of droplets can be analyzed. Samples are partitioned into ‘droplets’ then large quantities (~20,000 of these partitioned droplets are analyzed using microfluidics technology to provide incomparable precision). Only the positive fraction of the sample is detected; therefore, dPCR can detect mutant alleles in circulating tumor DNA, events as few as 0.01% of cells such as L1 insertions, low-level pathogens, and relative expression in single cells.

Applications

  • Copy number variant analysis

  • Gene expression analysis

  • SNP genotyping

  • Rare variant detection

  • Cancer biomarker discovery

  • Infectious diseases

  • Genomic alterations

  • Pathogen detection and load determination

Services

CGMP/GLP Compliant
Genomics & Biological Services

  • Experienced scientists functioning as project managers or study directors
  • Independent quality unit assuring regulatory compliance
  • Methods validated per ICH GLP/GMP guidelines – also research grade testing available
  • Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination
  • Controlled laboratory environment to prevent cross-contamination

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Thank you so much for successfully completing this important project for us on time. We are very satisfied with the final report and thank you for taking the extra effort to customize the report format. We will definitely use your lab for future projects.

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Dedication to Quality

Dedication To Quality

The Avance Biosciences team is committed to strict adherence to cGMP and GLP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

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Extensive Experience

Open Communication

Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

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Open Communication

Open Communication

Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

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Cutting Edge Science

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Avance Biosciences’ cGMP/GLP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, ddPCR, Sanger sequencing, next-gen sequencing, Southern blot, and more.

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