Next-gen amplicon sequencing is a powerful tool for biomarker research and diagnostic assay development.
Amplicon sequencing is a highly targeted approach that enables researchers to analyze genetic variation in specific genomic regions. The ultra-deep sequencing of PCR products (amplicons) allows efficient variant identification and characterization. This method uses oligonucleotide probes designed to target and capture regions of interest, followed by next-gen sequencing. Amplicon sequencing is useful for the discovery of rare somatic mutations in complex samples (such as tumors mixed with germline DNA).
Avance Biosciences offers three different amplicon sequencing approches with a focus on scalability, speed, and cost. State-of-the-art Illumina MiSeq platform is used. GLP/GMP-compliant service is available.