As part of our GLP/GMP Cell Bank Characterization services, Southern blot analysis is used evaluate the number of integration site and the expression construct structural integrity. Both integration site number and structure will be evaluated and may be compared to a reference if available.
DNA will be extracted from the test article, non-transfected host cells, followed by UV quantitation and electrophoresis analysis. After restriction digest with up to 4 enzymes, which cut once within the expression system, DNA samples are separated by electrophoresis and then transferred and UV cross-linked to a positive charged nylon membrane. Probe DNA will be prepared and labeled with either Chemiluminescent or 32P chemistry. After hybridization, the captured fragments will be visualized by exposure of X-Ray film. After producing the autoradiogram, the estimation of integration site number and the size of transgene will be reported.
Genomics & Biological Services
All Avance Services Include
Experienced scientists functioning as project managers or study directors
Controlled laboratory environment to prevent cross-contamination
Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination
Methods validated per ICH GLP/GMP guidelines – also research grade testing available
Projects customized and tailored to suit your needs
Independent quality unit assuring regulatory compliance
Transparent process flow and weekly report on project progress
Professional project reports ready for regulatory filing
Fast turn-around time & Competitive pricing