Southern blot services

Avance Biosciences offers Southern blot analysis to support gene discovery and confirmation, gene mapping, evolution and development studies, diagnostics, and forensics tests. Services are offered in compliance with GMP and GLP regulations promulgated by US FDA, European, and other regulatory agencies worldwide. Research grade Southern blot service is also available.
 Service Description
Screening for Vector Integration Southern blot analysis used to screen clones to confirm the effective and accurate integration of a transgene or a knock-out gene.
Structure Analysis by Southern Blot Restriction enzyme digestion of cell line DNA followed by Southern blotting is used to evaluate genetic stability of the integration site of an expression construct in a cell bank. Both integration site number and structure will be determined and may be compared to a reference if available.
Residual DNA Size Distribution Study This service is offered to detect the presence of an expressed transcript containing a sequence of interest. The size of the transcript will be estimated and the presence of partial transcripts will be reported.

Process

DNA will be extracted from testing samples followed by UV quantitation and electrophoresis analysis. After restriction digest with enzymes, DNA samples are separated by agarose gel electrophoresis and then transferred and UV cross-linked to a positive charged nylon membrane. Probe DNA will be prepared and labeled with either Chemiluminescent or 32P chemistry. After hybridization, the captured fragments will be visualized by exposure of X-Ray film.

Features

  • Method is validated per ICH guidelines
  • Both Radiative and chemiluminescent detection chemistries are offered
  • Staff is experienced in offering both GMP and GLP genomic services
  • Professional project report is ready for regulatory submissions
  • Turn-around time is 2 – 4 weeks
  • Excellent customer service focuses on client communication
  • Price is competitive

 

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We are dedicated to timely, high-quality service from the start of your project to the finish. Send us a quick message with your questions to find out what Avance can do for you.

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