We provide marker retention, lytic and lysogenic phage detection, bacterial viability determination, strain identity testing, purity testing, API 20E identification, and gram staining. Additional testing is available. We also provide extensive sequencing methodology, restriction digest analyses, and Southern and Northern blot testing.
Bacteria ID by API 20E | The API 20E test kit from bioMerieux, Inc, in addition to the oxidase test are offered for the identification of E. Coli and other enteric bacteria at the species level. |
Bacteriophage Testing | This service tests for the presence or absence of lytic phage contaminants in bacterial cell banks. |
Bacteriophage Titer Determination | If bacteriophages are present in a sample, the concentration of phages can be determined by evaluating a series of dilutions of the samples. |
Cell Culture Morphology Test | Test sample is plated and colony morphology is observed and identified using an electronic microscope |
Gram Staining | Bacteria are stained with dyes to differentiate Gram-positive bacterias from Gram-negative bacteria. Gram stain and cell shape will both be utilized for sample identity and purity. |
Microbial Cell Viability Test | Cell line sample is stained with trypan blue and the percentage of viable cells is calculated from the number of viable cells counted in relation to the total number of cells observed. |
Retention of Selectable Marker | This service determines the percentage of cells in a cell sample that contains a selected marker, such as an antibiotic-resistant gene. |