Real-Time PCR (RT PCR), or quantitative PCR (QPCR), is a DNA sequence detection and quantitation method extensively used for DNA copy number detection, gene expression, SNP genotyping, adventitious agent detection, and many other applications in the fields of genomics research, drug R&D, and biologics testing. Equipped with the state-of-art ABI 7900 HT Sequence Detection System and the QuantStudio 12K Flex Real-Time PCR System, Avance Biosciences offers both Real-Time PCR or End-Point PCR assays.
Absolute Quantitation Assay | Real-time PCR assay with absolute quantitation determined via a Standard Curve. |
Relative Quantitation Assay | This real-time PCR assay determines the fold-changes of the expression level of a gene relative to a calibrator or normalizing sample. |
Allelic Discrimination Assay | End-point PCR assay used to determine the genotype (homozygotes or heterozygotes) of samples. |
Plus/Minus Assay | End-point PCR assay used to determine the presence or absence of a specific target sequence in a sample. |