Digital PCR (dPCR) provides precise and absolute quantification of nucleic acids without a standard curve and without dependence on amplification efficiency.
Digital PCR is ultrasensitive; therefore, appropriate for testing allelic variants and targets of low abundance that are below the sensitivity limit of QPCR. It is also very precise, and is a better tool than QPCR when precise determination of the ratio of several nucleic acid sequences is required.
By employing multiple wells per sample, millions of droplets can be analyzed. Samples are partitioned into ‘droplets’ then large quantities (~20,000 of these partitioned droplets are analyzed using microfluidics technology to provide incomparable precision). Only the positive fraction of the sample is detected; therefore, dPCR can detect mutant alleles in circulating tumor DNA, events as few as 0.01% of cells such as L1 insertions, low-level pathogens, and relative expression in single cells.
Applications
Copy number variant analysis
Gene expression analysis
SNP genotyping
Rare variant detection
Cancer biomarker discovery
Infectious diseases
Genomic alterations
Pathogen detection and load determination
CGMP/GLP Compliant
Genomics & Biological Services
- Experienced scientists functioning as project managers or study directors
- Independent quality unit assuring regulatory compliance
- Methods validated per ICH GLP/GMP guidelines – also research grade testing available
- Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination
- Controlled laboratory environment to prevent cross-contamination
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When you partner with Avance Biosciences™, you gain a CRO partner that is creative, collaborative and dedicated to sound science with a focus on the regulatory requirements our partners require. Contact our technical staff to discuss how we can support you in your project!
