Avance Biosciences offers mRNA-Seq service using Illumina next generation sequencing platform to identify all mRNA transcripts expressed in a biological sample and precisely measure the relative quantities of each transcript including each isoform having different splicing patterns.

Biological samples (tissues, blood, etc) preserved for RNA extraction and total RNAs (RIN>7, 3 μg) extracted by the customer can be submitted for analysis. We will not accept extracted mRNA samples due to the difficulty in assessing their qualities. Sample species should be well characterized with reasonably complete genome information as well as transcript annotation.

The Illumina next generation sequencing platform and its associated mRNA-Seq protocols will be used for analysis.  In summary, messenger RNA with polyA tails will be purified from the total RNA samples. The mRNA will be fragmented and transcribed into ds cDNA for library preparation. Sequencing of the library will be done in a paired-end mode with a read length of 75 cycles.  The paired-end reads will be mapped to the genome, and the potential splicing pattern will be analyzed.  When applicable, expression levels of transcripts, genes, CDSs will be estimated, in RPKM, and reported.

Next-GenSequencing

NGS has the potential to unlock causative factors from our genetic material. Avance Biosciences is offering GLP/GMP next generation DNA Sequencing services using Illumina’s state-of-art MiSeq and HiSeq systems.
Services

GLP/GMP Compliant
Genomics & Biological Services

  • Experienced scientists functioning as project managers or study directors

  • Independent quality unit assuring regulatory compliance

  • Methods validated per ICH GLP/GMP guidelines – also research grade testing available

  • Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination

  • Controlled laboratory environment to prevent cross-contamination

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Our Customers Say…

From the preliminary conclusion of this work, we are already pleased to thank you for your customer-oriented approach and proactive communication in the course of this study. We are happy to see the profound difference in business mindset between your organization and our previous vendor…

Project Manager, Belgium

Thank you so much for successfully completing this important project for us on time. We are very satisfied with the final report and thank you for taking the extra effort to customize the report format. We will definitely use your lab for future projects.

Program Manager, California

It has been a pleasure working with you and your team. The project was carried out to the highest standard and delivered in a timely manner. I will definitely recommend Avance Biosciences’ services to colleagues and friends.

Senior Scientist, Singapore

When you partner with Avance Biosciences, you gain a CRO partner that is creative, collaborative and dedicated to sound science with a focus on the regulatory requirements our partners require. Contact our technical staff to discuss how we can support you in your project!

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Dedication to Quality

Dedication To Quality

The Avance Biosciences team is committed to strict adherence to CGMP and GLP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

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Extensive Experience

Open Communication

Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

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Open Communication

Open Communication

Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

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Cutting Edge Science

Open Communication

Avance Biosciences’ GLP/GMP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, ddPCR, Sanger sequencing, next-gen sequencing, Southern blot, and more.

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