Northern Blot Analysis Services
Northern blot analysis is a pivotal technique in the evaluation of cell banks utilized in drug development, Typically, MCB, WCB, and EOPC along with negative and positive controls are tested to support cell bank genetic stability. By employing Northern blotting, researchers can verify the integrity and consistency of mRNA transcripts, ensuring that the cell banks express the desired genes correctly and at appropriate levels.
Our Expertise
Avance Biosciences team has a long history of providing Northern blot analysis to support cell bank characterization for protein therapeutics development and manufacturing. Radioactive (P32) labeled hybridization probes are used in our lab for blotting and a Typhon FLA 7000 phosphorimager is used for detection. The quality of our blots is second to none, especially when considering most of our blots are done without the need of optimization.
Application
Integrated Gene Confirmation: mRNA extracted from integrated cells will be analyzed using northern blot to confirm the size of the integrated expression constructs.
GMP QC Analysis Process
- Assay Development: A gene-specific hybridization probe will be prepared by PCR from the provided control plasmid and optionally confirmed by Sanger Sequencing. A pilot study will be conducted with the P32-labeled probe following our standard Southern blot procedure that has been validated as a platform method.
- Assay Validation: A partial validation will be performed to demonstrate the specificity of the assay (probe specificity), and sensitivity of the blots.
- Sample Testing: MCB, WCB, and EOPC along with negative and positive controls are tested to support cell bank genetic stability.