Transcript Analysis by Northern Blot service is offered to detect the presence of an expressed transcript containing a sequence of interest. The size of the transcript will be estimated and the presence of partial transcripts will be reported.
Total RNA will be isolated from the test article (integrated cells) and untransfected cells. The total RNA (approximately 2 – 5 µg per sample) will be electrophoresed and transferred/cross-linked to positive-charged nylon membrane. The same probe designed for the Southern blots (if Southern blot analysis is performed) will be used for the Northern blots hybridization. Hybridized transcript(s) will be visualized by exposure of X-Ray film. After producing the autoradiogram, the size of the detected transcript(s) will be determined. A report with northern blot image and film, and transcript sizes will be delivered.
Avance Biosciences, Inc. provides all necessary expertise for Southern or Northern blot projects from nucleotide extraction to probe design. We offer Southern blot analysis to support gene discovery and confirmation, gene mapping, evolution and development studies, diagnostics, and forensics tests.
GLP/GMP Compliant
Genomics & Biological Services
Experienced scientists functioning as project managers or study directors
Independent quality unit assuring regulatory compliance
Methods validated per ICH GLP/GMP guidelines – also research grade testing available
Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination
Controlled laboratory environment to prevent cross-contamination
When you partner with Avance Biosciences, you gain a CRO partner that is creative, collaborative and dedicated to sound science with a focus on the regulatory requirements our partners require. Contact our technical staff to discuss how we can support you in your project!
