Transcript Analysis by Northern Blot service is offered to detect the presence of an expressed transcript containing a sequence of interest. The size of the transcript will be estimated and the presence of partial transcripts will be reported.

Total RNA will be isolated from the test article (integrated cells) and untransfected cells. The total RNA (approximately 2 – 5 µg per sample) will be electrophoresed and transferred/cross-linked to positive-charged nylon membrane. The same probe designed for the Southern blots (if Southern blot analysis is performed) will be used for the Northern blots hybridization. Hybridized transcript(s) will be visualized by exposure of X-Ray film. After producing the autoradiogram, the size of the detected transcript(s) will be determined. A report with northern blot image and film, and transcript sizes will be delivered.

Have questions?

We are dedicated to timely, high-quality service from the start of your project to the finish. Send us a quick message with your questions to find out what Avance can do for you.

CONTACT US

Ready to get started?

Give us a little background on your needs and the goals of your project, and we’ll send a preliminary quote including: tasks, deliverables and a no-obligation cost estimate.

REQUEST A QUOTE
Services

GLP/GMP Compliant
Genomics & Biological Services

  • Experienced scientists functioning as project managers or study directors

  • Independent quality unit assuring regulatory compliance

  • Methods validated per ICH GLP/GMP guidelines – also research grade testing available

  • Rigorous sample tracking and handling procedures to prevent mistakes and cross-contamination

  • Controlled laboratory environment to prevent cross-contamination

When you partner with Avance Biosciences™, you gain a CRO partner that is creative, collaborative and dedicated to sound science with a focus on the regulatory requirements our partners require. Contact our technical staff to discuss how we can support you in your project!

Partner

Dedication to Quality

Dedication To Quality

The Avance Biosciences™ team is committed to strict adherence to CGMP and GLP regulations enacted by the US, European, Japanese, and other international regulatory agencies.

LEARN MORE

Extensive Experience

Open Communication

Our team has extensive knowledge and experience working with scientists, QA/QC professionals, and project managers from over 100 pharmaceutical and biotechnology companies and organizations throughout the world.

LEARN MORE

Open Communication

Open Communication

Transparency and integrity are two of our core values. Our well-trained, friendly, and professional study directors and project managers strive to complete your projects on-time with open communication channels and to your specifications.

LEARN MORE

Cutting Edge Science

Open Communication

Avance Biosciences™’ CGMP/GLP compliant genomic and microbial assays make use of the advanced technologies including real-time PCR, ddPCR, Sanger sequencing, next-gen sequencing, Southern blot, and more.

LEARN MORE