Digital PCR (dPCR) provides precise and absolute quantification of nucleic acids without a standard curve and without dependence on amplification efficiency.
Digital PCR is ultrasensitive; therefore, appropriate for testing allelic variants and targets of low abundance that are below the sensitivity limit of QPCR. It is also very precise, and is a better tool than QPCR when precise determination of the ratio of several nucleic acid sequences is required.
By employing multiple wells per sample, millions of droplets can be analyzed. Samples are partitioned into ‘droplets’ then large quantities (~20,000 of these partitioned droplets are analyzed using microfluidics technology to provide incomparable precision). Only the positive fraction of the sample is detected; therefore, dPCR can detect mutant alleles in circulating tumor DNA, events as few as 0.01% of cells such as L1 insertions, low-level pathogens, and relative expression in single cells.
Genomics & Biological Services
Digital PCR Applications
Cancer biomarker discovery
Copy number variant analysis
Rare variant detection
Gene expression analysis
Pathogen detection and load determination
Absolute quantification of standards
Library quantification or clonal amplification of samples for next-generation sequencing
Characterization of low-fold changes in mRNA and miRNA expression
GMO detection and contamination assessment